Roles of calreticulin and calnexin during mucin synthesis in LS180 and HT29/A1 human colonic adenocarcinoma cells
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چکیده
منابع مشابه
Roles of calreticulin and calnexin during mucin synthesis in LS180 and HT29/A1 human colonic adenocarcinoma cells.
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin (CLN) during synthesis of two similar gastrointestinal mucins, MUC2 and MUC5AC. Pulse-chase labelling of MUC2 and MUC5A...
متن کاملSynthesis and secretion of mucin by the human colonic tumour cell line LS180.
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media without radiolabel for various lengths of time. From cell and media extracts obtained at each time point, mucin proteins were immunoprecipitated with specific anti-mucin antibodies and analysed by SDS/P...
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Lectins in Quality Control: Calnexin and Calreticulin
A long-standing enigma has been the role of N-linked glycans attached to many proteins in the endoplasmic reticulum (ER) and their coand posttranslational remodelling along the secretory pathway. Evidence is accumulating that intracellular animal lectins play important roles in quality control and glycoprotein sorting along the secretory pathway. Calnexin and calreticulin in conjunction with as...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1999
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3410593